解剖学报 ›› 2013, Vol. 44 ›› Issue (5 ): 607-611.doi: 10.3969/j.issn.0529-1356.2013.05.004

• 神经生物学 • 上一篇    下一篇

培养大鼠海马胆囊收缩素阳性神经元的电生理学特性

张阳慧1,2  魏晓菲2*  司军强1  马克涛1  张露露2  王钦文2   

  1. 1. 石河子大学医学院生理学教研室,新疆地方与民族高发病教育部重点实验室,新疆 石河子 832002;2. 宁波大学医学院生理学与药理学系,浙江省病理生理学技术研究重点实验室,浙江 宁波 315211
  • 收稿日期:2013-02-18 修回日期:2013-04-18 出版日期:2013-10-06 发布日期:2013-10-06
  • 通讯作者: 魏晓菲 E-mail:weixiaofei_ff@hotmail.com
  • 基金资助:

    国家自然科学基金资助项目;自然科学基金资助项目

Electrophysiological characteristics of cultured hippocampal cholecystokinin positive neurons

ZHANG Yang-hui 1,2  WEI Xiao-fei 2*  SI Jun-qiang1  MA Ke-taoZHANG Lu-lu2  WANG Qin-wen2   

  1. 1. Department of Physiology, Key Laboratory of Xinjiang Endemic and Ethnic DiseasesDepartment of Electrophysiology, Medical College of Shihezi University, Xinjiang Shihezi 832002,China;2. Department of Physiology and Pharmacology, Zhejiang Provincial Key Laboratory of Pathophysiology , School of Medicine, Ningbo University , Zhejiang Ningbo 315211, China
  • Received:2013-02-18 Revised:2013-04-18 Online:2013-10-06 Published:2013-10-06

摘要:

目的 探索海马内胆囊收缩素(CCK) 阳性(CCK+ )神经元的电生理学特性。方法 用整合有CCK启动子-绿色荧光蛋白(GFP)的腺病毒对培养海马CCK+神经元进行标记,应用全细胞膜片钳技术对标记CCK+神经元的电生理学特性进行研究。结果 CCK+神经元的胞体长径为(22.85±0.77)μm,短径为(16.21±0.42)μm (n=20),静息电位 (RMP)为(-55.90±1.30)mV,膜电容 (Cm)为(45.77±2.06)pF,膜阻抗 (Rm)为(711.00±46.69)MΩ; CCK+神经元500 ms内100 pA去极化电流诱发动作电位的发生频率为(26.17±3.41)Hz (n=12); CCK+神经元的自发抑制性突触后电流的发生频率[(5.26±0.71)Hz, n=7]显著高于自发兴奋性突触后电流的发生频率[(3.24±0.62)Hz, n=6]。 结论 CCK+神经元的相关电生理学特征可能与其在中枢神经系统内重要的调节功能相关。

关键词: 胆襄收缩素, 中间神经元, 海马, 全细胞膜片钳, 大鼠

Abstract:

Objective To explore the electrophysiological properties of cultured hippocampal cholecystokinin (CCK) positive (CCK+ ) neurons. Methods The cultured hippocampal CCK+ neurons were labeled by an adenovirus encoding CCK promoter and green fluorescent protein (GFP) and then whole cell patch clamp technique was carried on to explore the electrophysiology characteristic of hippocampal CCK+ neurons. Results The long diameter of CCK+ neurons’cell body was (22.85±0.77)μm, and the short diameter was (16.21±0.42)μm. The membrane electrophysiology parameters of CCK+ neurons were as following.
Rest membrane potential was(-55.90±1.30)mV. Membrane capacity was (45.77±2.06)pF. Membrane resistance was (711.00±46.69)MΩ. The action potential firing frequency of cultured hippocampal CCK+ neurons was (26.17±3.41)Hz. The spontaneous inhibitory postsynaptic currents frequency of CCK+ neurons was (5.26±0.71)Hz, which was significant higher compared to the frequency of spontaneous excitatory postsynaptic currents [(3.24±0.62)Hz]. Conclusion The electrophysiology characteristics of hippocampal CCK+ neuron may be colsely related to its modulatory effect in central nervous system.

Key words: Cholecystokinin, Interneuron, Hippocampus, Whole cell patch clamp, Rat